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magneticactivated cell sorting buffer mb  (Thermo Fisher)


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    Thermo Fisher magneticactivated cell sorting buffer mb
    Magneticactivated Cell Sorting Buffer Mb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sort+buffer/PHOSPHATE+BUFFERED+SALINE+PBS/pm41986506-431-5-24
    Average 99 stars, based on 1 article reviews
    magneticactivated cell sorting buffer mb - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Droplet-based single-cell joint profiling of histone modifications and transcriptomes.
    Article Snippet: The cell suspension was then filtered through a 30-μm Cell-Tric filter (Sysmex) for debris removal and centrifuged for 10 min at 300g at 4 °C. .. Cell pellets was washed once with douncing buffer, centrifuged again and resuspended in cold nuclei permeabilization buffer for 10 min. Permeabilized nuclei were pelleted by centrifugation for 10 min at 1,000g and 4 °C and washed with sort buffer (1× PBS (Gibco, 10010023), 1× protease inhibitor (Roche, 05056489001), 0.5 U μl−1 RNaseOUT (Invitrogen, 10777- 019), 0.5 U μl−1 SUPERaseIn inhibitor (Invitrogen, AM2694), 1 mM EDTA (Invitrogen, 15575020) and 1% bovine serum albumin (BSA; Sigma, A1595)) once. .. After resuspension in sort buffer, nuclei were stained with 2 μM 7-AAD (Invitrogen, A1310) for 10 min on ice and were sorted by fluorescence-activated nuclei sorting with an SH800 cell sorter (Sony) for the isolation of single nuclei (Extended Data Fig. 9).

    Article Title: Droplet-based single-cell joint profiling of histone modifications and transcriptomes
    Article Snippet: The cell suspension was then filtered through a 30-μm Cell-Tric filter (Sysmex) for debris removal and centrifuged for 10 min at 300 g at 4 °C. .. Cell pellets was washed once with douncing buffer, centrifuged again and resuspended in cold nuclei permeabilization buffer for 10 min. Permeabilized nuclei were pelleted by centrifugation for 10 min at 1,000 g and 4 °C and washed with sort buffer (1× PBS (Gibco, 10010023), 1× protease inhibitor (Roche, 05056489001), 0.5 U μl −1 RNaseOUT (Invitrogen, 10777-019), 0.5 U μl −1 SUPERaseIn inhibitor (Invitrogen, AM2694), 1 mM EDTA (Invitrogen, 15575020) and 1% bovine serum albumin (BSA; Sigma, A1595)) once. .. After resuspension in sort buffer, nuclei were stained with 2 μM 7-AAD (Invitrogen, A1310) for 10 min on ice and were sorted by fluorescence-activated nuclei sorting with an SH800 cell sorter (Sony) for the isolation of single nuclei (Extended Data Fig. ).

    Protease Inhibitor:

    Article Title: Droplet-based single-cell joint profiling of histone modifications and transcriptomes.
    Article Snippet: The cell suspension was then filtered through a 30-μm Cell-Tric filter (Sysmex) for debris removal and centrifuged for 10 min at 300g at 4 °C. .. Cell pellets was washed once with douncing buffer, centrifuged again and resuspended in cold nuclei permeabilization buffer for 10 min. Permeabilized nuclei were pelleted by centrifugation for 10 min at 1,000g and 4 °C and washed with sort buffer (1× PBS (Gibco, 10010023), 1× protease inhibitor (Roche, 05056489001), 0.5 U μl−1 RNaseOUT (Invitrogen, 10777- 019), 0.5 U μl−1 SUPERaseIn inhibitor (Invitrogen, AM2694), 1 mM EDTA (Invitrogen, 15575020) and 1% bovine serum albumin (BSA; Sigma, A1595)) once. .. After resuspension in sort buffer, nuclei were stained with 2 μM 7-AAD (Invitrogen, A1310) for 10 min on ice and were sorted by fluorescence-activated nuclei sorting with an SH800 cell sorter (Sony) for the isolation of single nuclei (Extended Data Fig. 9).

    Article Title: Droplet-based single-cell joint profiling of histone modifications and transcriptomes
    Article Snippet: The cell suspension was then filtered through a 30-μm Cell-Tric filter (Sysmex) for debris removal and centrifuged for 10 min at 300 g at 4 °C. .. Cell pellets was washed once with douncing buffer, centrifuged again and resuspended in cold nuclei permeabilization buffer for 10 min. Permeabilized nuclei were pelleted by centrifugation for 10 min at 1,000 g and 4 °C and washed with sort buffer (1× PBS (Gibco, 10010023), 1× protease inhibitor (Roche, 05056489001), 0.5 U μl −1 RNaseOUT (Invitrogen, 10777-019), 0.5 U μl −1 SUPERaseIn inhibitor (Invitrogen, AM2694), 1 mM EDTA (Invitrogen, 15575020) and 1% bovine serum albumin (BSA; Sigma, A1595)) once. .. After resuspension in sort buffer, nuclei were stained with 2 μM 7-AAD (Invitrogen, A1310) for 10 min on ice and were sorted by fluorescence-activated nuclei sorting with an SH800 cell sorter (Sony) for the isolation of single nuclei (Extended Data Fig. ).

    Recombinant:

    Article Title: Understanding cell fate acquisition in stem-cell-derived pancreatic islets using single-cell multiome-inferred regulomes.
    Article Snippet: Samples were incubated on a rotator for 5 min at 4 C and then centrifuged at 500g for 5 min (Eppendorf, 5920R; 4 C, ramp speed of 3/3). .. Supernatant was removed and pellet was resuspended in sort buffer [1mM EDTA (Invitrogen, 15575020), 0.2U/mL Recombinant RNAsin (Promega, PAN2515), 1% Fatty-acid-free BSA in PBS (Proliant, 7500804; Corning, 21-040-CV) and stained with DRAQ7 (1:150; Cell Signaling Technology, 7406). .. 60,000 nuclei were sorted using an SH800 sorter (Sony) into 50 ml of collection buffer [1.0U/mL Recombinant RNAsin (Promega, PAN2515), 5% Fatty-acid-free BSA in PBS (Proliant, 7500804; Corning, 21-040- CV)].

    Staining:

    Article Title: Understanding cell fate acquisition in stem-cell-derived pancreatic islets using single-cell multiome-inferred regulomes.
    Article Snippet: Samples were incubated on a rotator for 5 min at 4 C and then centrifuged at 500g for 5 min (Eppendorf, 5920R; 4 C, ramp speed of 3/3). .. Supernatant was removed and pellet was resuspended in sort buffer [1mM EDTA (Invitrogen, 15575020), 0.2U/mL Recombinant RNAsin (Promega, PAN2515), 1% Fatty-acid-free BSA in PBS (Proliant, 7500804; Corning, 21-040-CV) and stained with DRAQ7 (1:150; Cell Signaling Technology, 7406). .. 60,000 nuclei were sorted using an SH800 sorter (Sony) into 50 ml of collection buffer [1.0U/mL Recombinant RNAsin (Promega, PAN2515), 5% Fatty-acid-free BSA in PBS (Proliant, 7500804; Corning, 21-040- CV)].

    Article Title: IGHV1 usage is associated with lymphadenopathy and aggressive disease in the TCL1 mouse model for chronic lymphocytic leukemia
    Article Snippet: 2.0 × 10 7 – 2.2 × 10 7 viable cells were stained for 20 min at room temperature in PBS, using the following antibodies: anti-mouse CD5 BV421 (clone: 53 − 7.3); anti-mouse CD4 FITC (clone: RM4-4); anti-mouse CD19 APC (clone: 6D5) (Biolegend, San Diego, CA, USA); anti-mouse CD8a APC-H7 (clone: 53 − 6.7) (BD, Franklin Lakes, NJ, USA). .. After staining, cells were washed once with 2 ml PBS, resuspended in sort buffer (PBS supplemented with 2% FCS and 0.025 M HEPES (Gibco, Billings, MT, USA, cat: 15630-080), and CD5 + /CD19 + cells were sorted. ..



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